Biological Indicators

The Centers for Disease Control and Prevention recommend regular use of mechanical, chemical and biological indicators to ensure the effectiveness of sterilization processes.1 Clorox Healthcare/ Healthlink offers a variety of sterilization monitoring products to meet the needs of your practice.

ProSure® Mailers

Mail-in monitoring system

ProSure® Mailers are designed to provide third party verification that steam, chemical vapor, dry heat and EO gas sterilization processes are functioning properly.

After running a cycle with ProSure® strips, simply place the strips in the pre-addressed envelope and mail to HealthLink for processing. We will provide you with written results.

Use ProSure mailers weekly for sterilization monitoring or in conjunction with EZTestTM in-office monitoring system to validate in-office results.

  • Convenient pre-addressed mailers enable easy processing
  • Written results in 24 hours2

2. 24 hour results from receipt of enveloped by Healthlink. Dry heat results require 7 days.

3910ProSure® Mailer12/box
3926ProSure® Mailer26/box
3952ProSure® Mailer52/box

EZTestTM Steam

Plastic vial for steam sterilization cycles

EZTestTM Steam vials contain a liquid culture of Geobacillus sterathermophilus spores and are intended for monitoring the e cacy of steam sterilization cycles 121°C - 135°C.

  • Perform in-o ce testing at least weekly to monitor steam sterilizers
  • Fits incubators currently on the market3
  • Results in 24 hours

3. Fits 3MTM AttestTM incubators. Replaces 3MTM AttestTM 1261, 1261P, 1262 & 1262P * Starter Kit includes EZTestTM, ProPackTM Integrator and Incubator

3917EZTestTM Steam plastic vial25/box
3918EZTestTM Steam plastic vial100/box
3609EZTestTM Starter Kit (25/box)1
3610EZTestTM Starter Kit (100/box)1


Plastic vial for EO sterilization cycles

EZTestTM EO plastic vials include a paper disk carrier of Bacillus atrophaeus spores. The kit also includes media for growing the bacterial spores in glass vials.

  • Perform in-o ce testing at least weekly to monitor ethylene oxide sterilizers
  • Easy to read color-changing media (bright yellow= spore growth)
  • Results in 48 hours
3931EZTestTM EO plastic vial25/box
3932EZTestTM EO plastic vial100/box


Glass ampoule for steam sterilization of liquids

ProSpore ampoules contain a liquid culture of Geobacillus sterathermophilus spores, and are designed to monitor and validate liquid sterilization in steam sterilizers.

  • Perform in-o ce testing at least weekly to monitor steam sterilizers
  • Easy-to-process vials
  • Results in 48 hours
3471ProSpore glass ampoule10/box
3571ProSpore glass ampoule50/box

Bowie-Dick Type Test

Vacuum - Test Pack for Steam Sterilization

HealthLink® Vacuum Test Pack (a Bowie-Dick type test) is a disposable test used to evaluate the e cacy of the vacuum system of a steam sterilizer.

Performed daily, this test validates the performance of a steam chamber’s vacuum system by determining whether su cient air has been removed from the chamber prior to the introduction of steam.

  • Results are readable immediately after running the test
  • Designed for daily usage along with other Healthlink biological indicators
3975Bowie-Dick Test80/case
3980Bowie-Dick Test20/box


For in-office monitoring

Dry-bath aluminium block incubator for in-house monitoring. Designed for use with EZTestTM Steam and EZTestTM EO.

  • Small footprint
  • Includes LED thermometer
  • Built-in ampoule crasher
Operation Manual Download (pdf)
3613EZTestTM LED 35°C, 57°C, 60°C,
Model 1410, each
1. Rutala WA, Weber, DJ and the Healthcare Infection Control Practices Advisory Committee (HICPAC).Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008 infectioncontrol/guidelines/disinfection/ (Accessed 24 July 2017)